Lundi 24 août 2009
1
24
/08
/Août
/2009
17:12
Humans might not be walking the face of the Earth were it not for the ancient fusing of two
prokaryotes — tiny life forms that do not have a cellular nucleus. Endosymbiosis refers to a cell living within another cell. If the cells live together long enough, they will exchange genes;
they merge but often keep their own cell membranes and sometimes their own genomes.
"We have been overlooking how important cooperation is," UCLA molecular
biologist James A. Lake said. "If two prokaryotes get together, they can change the world. They restructured the atmosphere of the Earth. It's a message that evolution is giving us: Cooperation
is a way to get ahead."
Lake discovered the first exclusively prokaryote endosymbiosis. All other
known endosymbioses have involved a eukaryote — a cell that contains a nucleus. Eukaryotes are found in all multicellular forms of life, including humans, animals and
plants.
"This relationship resulted in a totally different type of life on Earth,"
said Lake, a UCLA distinguished professor of molecular, cell and developmental biology and of human genetics. "We thought eukaryotes always needed to be present to do it, but we were
wrong."
Lake reported that two groups of prokaryotes — actinobacteria and clostridia
— came together and produced "double-membrane" prokaryotes.
"Higher life would not have happened without this event," Lake said. "These
are very important organisms. At the time these two early prokaryotes were evolving, there was no oxygen in the Earth's atmosphere. Humans could not live. No oxygen-breathing organisms could
live."
The oxygen on the Earth is the result of a subgroup of these double-membrane
prokaryotes, Lake said. This subgroup, the cyanobacteria, used the sun's energy to produce oxygen through photosynthesis. They have been tremendously productive, pumping oxygen into the
atmosphere; we could not breathe without them. In addition, the double-membrane prokaryotic fusion supplied the mitochondria that are present in every human cell, he
said.
"This work is a major advance in our understanding of how a group of
organisms came to be that learned to harness the sun and then effected the greatest environmental change the Earth has ever seen, in this case with beneficial results," said Carl Pilcher,
director of the NASA Astrobiology Institute, headquartered at the NASA Ames Research Center in Moffett Field, Calif., which co-funded the study with the National Science
Foundation.
"Along came these organisms — the double-membrane prokaryotes — that could
use sunlight," Lake said. "They captured this vast energy resource. They were so successful that they have more genetic diversity in them than all other prokaryotes.
"We have a flow of genes from two different organisms, clostridia and
actinobacteria, together," he said. "Because the group into which they are flowing has two membranes, we hypothesize that that was an endosymbiosis that resulted in a double membrane. It looks as
if a single-membrane organism has engulfed another. The genomes are telling us that the double-membrane prokaryotes combine sets of genes from the two different
organisms."
For this study whuch will be published in Nature, Lake has looked back more
than 2.5 billion years. He conducted an analysis of the genomics of the five groups of prokaryotes.
Posted by Casey Kazan
Source: University of California - Los Angeles